A Rapid and Robust Sample Preparation Method for Quantitation of Nicotine from Oral Fluid

Abstract:

In this technical note, we present a solid phase extraction (SPE) method for simultaneous detection and quantitation of nicotine, cotinine, and anabasine from oral fluid. We employ Intercept i2®, a commercially available oral fluid collection device for sample collection and transport. A polymeric, strong cation-exchange sorbent, Strata®-X-C was utilized for solid phase extraction (SPE). pH stable Kinetex® 2.6 μm EVO C18 column was used for the analysis purposes to obtain the best selectivity between the two isomeric compounds nicotine and anabasine under an ESI mode in LC/MS/MS analysis.

Document Type:

Technical Notes

Industries:

Forensics/Toxicology

Techniques:

HPLC / UHPLC Sample Prep

Brands:

Strata-X Kinetex

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