Region

PREP and DAC

Scale Up Calculators

DAC Scale Up Calculator

Choose your Bulk Media and Particle Size
Media Density 0
Column Scout/Prep Column DAC Column
Length (mm)
Diameter (mm)
Column Volume (empty) (mL) 0 0
Column Bed Volume (empty) (mL) 0 0
Equilibration time (min) (10 C.V.) 0 0
Weight of packing mat (mg)* 0 0
Scaling Factor 0
Flow rate (mL/min) 0
Sample Amt (g) 0
Injection volume (mL) 0
Notes:
  • Back pressure stays constant if column length stays constant
  • Back pressure will increase with decrease in particle size
  • Efficiency will increase with decrease in particle size
  • With scale up change in hardware and flow cell may be required
* 0.6 g/mL was used

Visit us at Phenomenex.com/Products/Format/Axia

*Please note all calculations are approximations only. Several factors influence loading capacity of columns. For example:α , solubility, RT, surface area of material, sample type, instrument, etc.